Acc36 I

3,080.00 ₽ – 12,320.00 ₽

  • CNY: 236.67 ¥ - 946.67 ¥

Restriction endonuclease Acc36 I

  • Recognition site: ACCTGC(N)4↑ / TGGACG(N)8↓
  • Источник: Acinetobacter calcoaceticus 36
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E289 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E289S100 uRegular2 000 - 5 000 u/ml3,080.00 ₽
  • CNY: 236.67 ¥
E290L500 uRegular2 000 - 5 000 u/ml12,320.00 ₽
  • CNY: 946.67 ¥

Description

Recognition site and hydrolysis position:

ACCTGC(N)4 ACCTGC(N)4↑
TGGACG(N)8 TGGACG(N)8↓

Source: Acinetobacter calcoaceticus 36
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
25 25 50 50 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 4 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration may result in star activity
References:
Gonchar, D.A., Shinkarenko, N.M., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations. 1999

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