AspS9 I

4,000.00 16,000.00 

  • CNY: 311.28 ¥ - 1,245.12 ¥

Restriction endonuclease AspS9 I

  • Recognition site: G↑GNCC / CCNG↓G
  • Источник: Arthrobacter species S9
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E117 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E117TS1000 uTurbo20 000 u/ml4,000.00 
  • CNY: 311.28 ¥
E118TL5000 uTurbo20 000 u/ml16,000.00 
  • CNY: 1,245.12 ¥
E117S1000 uRegular20 000 u/ml4,000.00 
  • CNY: 311.28 ¥
E118L5000 uRegular20 000 u/ml16,000.00 
  • CNY: 1,245.12 ¥

Description

Recognition site and hydrolysis position:

GGNCC G↑GNCC
CCNGG CCNG↓G

Source: Arthrobacter species S9
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
50 50 75 100 50 75

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W
Reagents Supplied with Enzyme:
Methylation sensitivity: Bloked by overlapping Dcm methylation(CmCWGG): GGNCCWGGNotes:
References:
Dedkov, V.S., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1995).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006