AsuHP I

Description

Recognition sequence:

Source: An E.coli strain that carries the cloned AsuHPI gene from Actinobacillus suis HP
Unit Definition: One unit is defined as the amount of enzyme required to digest 1 µg of  λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl.
Optimal buffer: SE-buffer O
Enzyme activity (%):

Storage conditions: 10 mM Tris-HCl (pH 7.5), 250 mM NaCl, 0.1 mM EDTA, 7 mM 2-mercaptoethanol, 200 µg/ml BSA, 50% glycerol; Store at -20°С.
Ligation: After 5-fold overdigestion with AsuHP I, ~30% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Methylation sensitivity: Blocked by overlapping dam-methylation (GmATC): GGTGATCReagents Supplied with Enzyme: 10 x SE-Buffer O
Notes: enzyme may cleave at N9/N8 depending on the sequence between the ecognition and cleave sites.
References:

• Dedkov, V.S., Degtyarev, S.Kh. Biol. Chem. 379: 573-574 (1998)
• Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
• Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // “Medical genetics” V.6, No 8, pp 29-36, 2007

Dedkov, V.S., Degtyarev, S.Kh. Biol. Chem. 379: 573-574 (1998).
Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // Translated from “Medical genetics” V.6, No 8, pp 29-36, 2007