Bar I

5,500.00 ₽ – 22,000.00 ₽

  • CNY: 419.54 ¥ - 1,678.16 ¥

Restriction endonuclease Bar I

  • Recognition site: ↑(N)7GAAGNNNNNNTAC(N)12↑ / ↓(N)12CTTCNNNNNNATG(N)7↓
  • Источник: Bacillus sphaericus
  • Оптимальный буфер: SE-буфер 2K
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E547 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E547S100 uRegular500-2 000 u/ml5,500.00 ₽
  • CNY: 419.54 ¥
E548L500 uRegular500-2 000 u/ml22,000.00 ₽
  • CNY: 1,678.16 ¥

Description

Recognition site and hydrolysis position:

(N)7GAAGNNNNNNTAC(N)12 ↑(N)7GAAGNNNNNNTAC(N)12↑
(N)12CTTCNNNNNNATG(N)7 ↓(N)12CTTCNNNNNNATG(N)7↓

Source: Bacillus sphaericus
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
T7 DNA
Optimal SE-buffer: SE-buffer 2K
Enzyme activity (%):

B G O W Y ROSE
0 0 25 50 10 40

Storage conditions: 20 mM KH2PO4(pH 7.4); 100 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
Ligations:
After 2-fold overdigestion with enzyme 90% of DNA fragments can be ligated. Of these 95% can be recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer 2K
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:

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