Bgl I

1,725.00 ₽ – 6,900.00 ₽

  • CNY: 133.36 ¥ - 533.44 ¥

Restriction endonuclease Bgl I

  • Recognition site: GCCNNNN↑NGGC / CGGN↓NNNNCCG
  • Источник: Bacillus globigii
  • Оптимальный буфер: SE-буфер 2W
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E025 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E025TS500 uTurbo5 000-10 000 u/ml1,725.00 ₽
  • CNY: 133.36 ¥
E026TL2500 uTurbo5 000-10 000 u/ml6,900.00 ₽
  • CNY: 533.44 ¥
E025S500 uRegular5 000-10 000 u/ml1,725.00 ₽
  • CNY: 133.36 ¥
E026L2500 uRegular5 000-10 000 u/ml6,900.00 ₽
  • CNY: 533.44 ¥

Description

Recognition site and hydrolysis position:

GCCNNNNNGGC GCCNNNN↑NGGC
CGGNNNNNCCG CGGN↓NNNNCCG

Source: Bacillus globigii
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer 2W
Enzyme activity (%):

B G O W Y ROSE
50 50 0 75 25 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer 2W
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by dcm methylation (CmCWGG): GCCWGGNNGGCNotes: High enzyme concentration may result in star activity
References:
Duncan, C.H., Wilson, G.A. and Young, F.E. J. Bacteriol. 134: 338 (1978).

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