Bpu14 I

1,495.00 ₽ – 5,980.00 ₽

  • CNY: 114.46 ¥ - 457.83 ¥

Restriction endonuclease Bpu14 I

  • Recognition site: TT↑CGAA / AAGC↓TT
  • Источник: Bacillus pumilus 14
  • Оптимальный буфер: SE-буфер G
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E033 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E033TS1000 uTurbo10 000-20 000 u/ml1,495.00 ₽
  • CNY: 114.46 ¥
E034TL5000 uTurbo10 000-20 000 u/ml5,980.00 ₽
  • CNY: 457.83 ¥
E033S1000 uRegular10 000-20 000 u/ml1,495.00 ₽
  • CNY: 114.46 ¥
E034L5000 uRegular10 000-20 000 u/ml5,980.00 ₽
  • CNY: 457.83 ¥

Description

Recognition site and hydrolysis position:

TTCGAA TT↑CGAA
AAGCTT AAGC↓TT

Source: Bacillus pumilus 14
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer G
Enzyme activity (%):

B G O W Y ROSE
50 100 25 25 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
Ligations:
After 10-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Semenchenko, G.V., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations (1994).

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