BstEN I

1,150.00 ₽ – 4,600.00 ₽

  • CNY: 88.37 ¥ - 353.46 ¥

Restriction endonuclease BstEN I

  • Recognition site: CCTNN↑NNNAGG / GGANNN↓NNTCC
  • Источник: Bacillus stearothermophillus EN
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 65
  • Температура инактивации, 20 мин при: 80
SKU: SE-E103 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E103S200 uRegular2 000 - 5 000 u/ml1,150.00 ₽
  • CNY: 88.37 ¥
E104L1000 uRegular2 000 - 5 000 u/ml4,600.00 ₽
  • CNY: 353.46 ¥

Description

Recognition site and hydrolysis position:

CCTNNNNNAGG CCTNN↑NNNAGG
GGANNNNNTCC GGANNN↓NNTCC

Source: Bacillus stearothermophillus EM
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
50 50 25 25 100 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut. Of these 90% can be recut
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 65°C Reagents Supplied with Enzyme 10 X SE-buffer Y
Reagents Supplied with Enzyme:
Methylation sensitivity: Not blocked by overlapping Dcm methylation(CmCWGG): CCTGGNNNAGG,
CCTNNNCCAGG.
Notes:
References:
Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000).

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