Cci I

4,400.00 ₽ – 17,600.00 ₽

  • CNY: 336.86 ¥ - 1,347.46 ¥

Restriction endonuclease Cci I

  • Recognition site: T↑CATGA / AGTAC↓T
  • Источник: Curtobacterium citreus
  • Оптимальный буфер: SE-буфер W
  • Оптимальная температура: 55
  • Температура инактивации, 20 мин при: 80
SKU: SE-E565 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E565TS1000 uTurbo20 000 u/ml4,400.00 ₽
  • CNY: 336.86 ¥
E566TL5000 uTurbo20 000 u/ml17,600.00 ₽
  • CNY: 1,347.46 ¥
E565S1000 uRegular20 000 u/ml4,400.00 ₽
  • CNY: 336.86 ¥
E566L5000 uRegular20 000 u/ml17,600.00 ₽
  • CNY: 1,347.46 ¥

Description

Recognition site and hydrolysis position:

TCATGA T↑CATGA
AGTACT AGTAC↓T

Source: Curtobacterium citreus
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer W
Enzyme activity (%):

B G O W Y ROSE
0 10 25 100 75 100

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of DNA with 40 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer W, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: Do not use BSA for long incubation
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
References:

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