EcoR I

1,650.00 6,600.00 

  • CNY: 128.40 ¥ - 513.61 ¥

Restriction endonuclease EcoR I

  • Recognition site: G↑AATTC / CTTAA↓G
  • Источник: Из штамма E.coli несущего клонированный ген EcoR I из Escherichia coli
  • Оптимальный буфер: SE-буфер EcoRI
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E057 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E057S5000 uRegular20 000 u/ml1,650.00 
  • CNY: 128.40 ¥
E058L25000 uRegular20 000 u/ml6,600.00 
  • CNY: 513.61 ¥
E057XS5000 uConcentrated50 000 u/ml1,650.00 
  • CNY: 128.40 ¥
E058XL25000 uConcentrated50 000 u/ml6,600.00 
  • CNY: 513.61 ¥
E057TS5000 uTurbo20 000 u/ml1,650.00 
  • CNY: 128.40 ¥
E058TL25000 uTurbo20 000 u/ml6,600.00 
  • CNY: 513.61 ¥

Description

Recognition site and hydrolysis position:

GAATTC G↑AATTC
CTTAAG CTTAA↓G

Source: An E.coli strain that carries the cloned EcoR I gene from Escherichia coli
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer EcoRI
Enzyme activity (%):

B G O W Y ROSE
50 75 75 75 50 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 40-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer EcoRI, BSA
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration and using of nonoptimal buffer may result in star activity.
Do not use BSA for long incubation.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
References:
Albertsen, H.M., Le Paslier, D., Abderrahim, H., Dausset, J., Cann, H., Cohen, D. Nucleiv Acids Res. 17: 808 (1989).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006