Hpa I

2,530.00 10,120.00 

  • CNY: 196.88 ¥ - 787.54 ¥

Restriction endonuclease Hpa I

  • Recognition site: GTT↑AAC / CAA↓TTG
  • Источник: Из штамма E.coli несущего клонированный ген Hpa I из Haemophilus parainfluenzae
  • Оптимальный буфер: SE-буфер Y
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E077 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E077S500 uRegular5 000 u/ml2,530.00 
  • CNY: 196.88 ¥
E078L2500 uRegular5 000 u/ml10,120.00 
  • CNY: 787.54 ¥
E077TS500 uTurbo5 000 u/ml2,530.00 
  • CNY: 196.88 ¥
E078TL2500 uTurbo5 000 u/ml10,120.00 
  • CNY: 787.54 ¥

Description

Recognition site and hydrolysis position:

GTTAAC GTT↑AAC
CAATTG CAA↓TTG

Source: An E.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer Y
Enzyme activity (%):

B G O W Y ROSE
0 50 10 25 100 25

Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
Ligations:
After 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes: High enzyme concentration results in star activity.
References:
Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973). Garfin, D.E., Goodman, H.M. Biochem. Biophys. Res. Commun. 59: 108-116 (1974).