Mlu I

2,970.00 ₽ – 11,880.00 ₽

  • CNY: 229.61 ¥ - 918.44 ¥

Restriction endonuclease Mlu I

  • Recognition site: A↑CGCGT / TGCGC↓A
  • Источник: Micrococcus luteus
  • Оптимальный буфер: SE-буфер O
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: 65
SKU: SE-E085 Category:

Available Options

SKUРackagePackageVariantConcentrationPrice 
E085S1000 uRegular20 000 u/ml2,970.00 ₽
  • CNY: 229.61 ¥
E086L5000 uRegular20 000 u/ml11,880.00 ₽
  • CNY: 918.44 ¥
E085TS1000 uTurbo10 000-30 000 u/ml2,970.00 ₽
  • CNY: 229.61 ¥
E086TL5000 uTurbo10 000-30 000 u/ml11,880.00 ₽
  • CNY: 918.44 ¥

Description

Recognition site and hydrolysis position:

ACGCGT A↑CGCGT
TGCGCA TGCGC↓A

Source: Micrococcus luteus
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer O
Enzyme activity (%):

B G O W Y ROSE
0 10 100 25 10 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
Ligations:
After 20-fold overdigestion with enzyme 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O.
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Notes:
References:
Sugisaki, H., Kanazawa, S. Gene 16: 73-78 (1981).

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