Rsa I

3,600.00 14,400.00 

  • CNY: 31.59 ¥ - 126.35 ¥

Restriction endonuclease Rsa I

  • Recognition site: GT↑AC / CA↓TG
  • Источник: Rhodopseudomonas sphaeroides
  • Оптимальный буфер: SE-буфер B
  • Оптимальная температура: 37
  • Температура инактивации, 20 мин при: N
SKU: SE-E113 Category:


Recognition site and hydrolysis position:


Source: Rhodopseudomonas sphaeroides
Unit definition:
One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Assayed on:
Lambda DNA
Optimal SE-buffer: SE-buffer B
Enzyme activity (%):

100 50 0 50 75 50

Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C.
After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisis:
No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer B
Reagents Supplied with Enzyme:
Methylation sensitivity: not tested
Lynn, S.P., Cohen, L.K., Kaplan, S., Gardner, J.F. J.Bacteriol. 142: 380-383 (1980).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // Translated from “Ovchinnikov bulletin of biotechnology and physical and chemical biology” V.2, No 3, pp 39-46, 2006

Available Options

SKUРackage sizePackageVariantConcentrationPrice 
E113TS1000 uTurbo10 000 u/ml3,600.00 
E114TL5000 uTurbo10 000 u/ml14,400.00 
E113S1000 uRegular10 000 u/ml3,600.00 
E114L5000 uRegular10 000 u/ml14,400.00