Uracil DNA Glycosylase

3,520.00 14,080.00 

  • CNY: 273.01 ¥ - 1,092.04 ¥

  • Источник: Из штамма E.coli, несущего рекомбинантный ген
  • Оптимальный буфер: SE-буфер Урацил ДНК гликозилаза
  • Оптимальная температура: 37
SKU: SE-E335 Category:

Available Options

SKUРackagePackageConcentrationPrice 
E335S1000 u20 000-50 000 u/ml3,520.00 
  • CNY: 273.01 ¥
E336L5000 u20 000-50 000 u/ml14,080.00 
  • CNY: 1,092.04 ¥

Description

E.coli uracil-DNA glycosylase (UDG) catalyzes the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases)
Applications:
– site-directed mutagenesis;
– as a probe for protein-DNA interaction studies;
Treatment of 1 μg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95°C for 10 minutes.

Source: Isolated from the recombinant source

Unit definition: E.coli uracil-DNA glycosylase (UDG) catalyzes the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases)
Applications:
– site-directed mutagenesis;
– as a probe for protein-DNA interaction studies;
Treatment of 1 μg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95°C for 10 minutes.

Reaction buffer: SE-buffer Uracil DNA Glycosylase

Storage conditions: 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.

Quality control: