Product info: Aat II


Name
Aat II
Cat. #E287E288
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition site
GACGTC
CTGCAG
SourceE.coli strain, that carries the cloned Aat II gene from Acetobacter aceti
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10 - 2525 - 5010 - 2525 - 5010050
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesHigh enzyme concentration may result in star activity.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Sugisaki H., Maekawa Y., Kanazawa S., Takanami M. Nucleic Acid Res. 10:5747-5752(1982)
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322