Product info: BstAF I


Name
BstAF I
Cat. #E135E136
Package, u.a.10005000
Concentration, u.a./ml2000020000

Recognition site
CTTAAG
GAATTC
SourceBacillus stearothermophilus AF
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
10 - 2525 - 5075 - 10010025 - 50100
Optimal temperature
55oC
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme 40% of the DNA fragments can be ligated and recut. In the presence of 10% PEG ligation is better.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 55°C.
Reagents Supplied with Enzyme 10 X SE-buffer W
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
NotesDo not use BSA for long incubation.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322