Product info: Gsa I


Name
Gsa I
Cat. #E563E564
Package, u.a.10005000
Concentration, u.a./ml10000-2000010000-20000

Recognition site
CCCAGC
GGGTCG
SourceGeobacillus stearothermophilus Y
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 70°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
10 - 2525 - 5075 - 10010075 - 100100
Optimal temperature
70oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme about 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of DNA with 20 u.a. of enzyme for 16 hours at 70°C.
Reagents Supplied with Enzyme 10 X SE-buffer W, BSA
Methylation sensitivitynot tested
Inactivation 20 minutes
No
NotesDo not use BSA for long incubation.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Dedkov V.S., Mikhnenkova N.A., Kileva E.V., Tarasova G.V., Gonchar D.A., Akishev A.G., Chernukhin V.A., Shkirja S.S., Degtyarev S.Kh.
New restriction endonuclease GsaI, neoschizomer of BseY I, recognizes 5-CCCAGC(-1/-5)-3.// Bulletin of biotechnology and physico-chemical biology named by Yu.A.Ovchinnikov (Moscow), V.4, No.1, p.19-24 (2008). (In Russian)
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322