| Source | IIsolated from E.coli strain that carries the cloned Taq DNA polymerase gene from Thermus aquaticus. |
| Description | Taq DNA polymerase is a thermostable DNA polymerase that possesses a 5` -> 3` polymerase activity and a double strand specific 5` -> 3` exonuclease activity.
Applications:
- nick translation;
- primer extension reaction.
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| Unit definition | One unit is the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.
Unit Assay Conditions: 1 x AS buffer, 200 µM dNTPs including [3H]-dTTP and 250 µg/ml activated calf thymus DNA.
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| Reaction buffer | SE-buffer AS (Ammonium Sulfate), (67 mM Tris-HCl (pH 8.8 at 25°C); 16.6 mM (NH4)2SO4, 0.01 % Tween-20. Supplementary material is 50 mM MgCl2.) |
| Optimal temperature | 72oC |
| Storage conditions | 20 mM Tris-HCl (pH 7.6); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% Triton X-100; 50% glycerol. Store at -20°C. |
| Quality control | E.coli DNA free.
The enzyme is purified free of contaminating endonucleases and
exonucleases.
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| Legal |
Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States, European Union and certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used. |
| MSDS: | Download MSDS as PDF |