Product info: Ars I


Name
Ars I
Cat. #E575E576
Package, u.a.50250
Concentration, u.a./ml500500

Recognition site
(N)8GACNNNNNNTTYG(N)11
(N)13CTGNNNNNNAARC(N)6
SourceArthrobacter species NTS
Assayed onT7 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 30°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
0 - 100 - 100 - 100 - 1010020
Optimal temperature
30oC
Storage conditions10 mM KH2PO4(pH 7.4); 200 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol. Store at -20°C.
LigationsAfter 3-fold overdigestion with enzyme 70% of DNA fragments can be ligated. Of these 80% can be recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 1 u.a. of enzyme for 16 hours at 30°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesTo obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF