Product info: Bsu I


Name
Bsu I
Cat. #E581E582
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

Recognition site
GTATCC(N)6
CATAGG(N)5
SourceBacillus sphaericus
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10050 - 7510 - 2525 - 5010010
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 0.05% Triton X-100, 50% glycerol; Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme 10% of DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesWhen using a buffer other than the optimal (suppied) SEBuffer, it may be necessary to add more enzyme to achieve complete digestion.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF