Recognition site | CAGNNN↑CTG GTC↓NNNGAC |
Source | Pseudomonas stutzeri 217 |
Assayed on | Lambda DNA |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
Enzyme activity (%) | B | G | O | W | Y | Rose | 25 - 50 | 50 - 75 | 10 - 25 | 25 - 50 | 100 | 100 |
|
Optimal temperature | 37oC |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at -20°C. |
Ligation | After 5-fold overdigestion with enzyme > 95% of Lambda DNA fragments can be ligated with T4 DNA Ligase and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
|
Reagents Supplied with Enzyme |
10 X SE-buffer Y |
Methylation sensitivity | not tested |
Inactivation 20 minutes under | 65oC |
Notes | When using a buffer other than the
optimal (suppied) SEBuffer, it may be
necessary to add more enzyme to achieve complete digestion.
|
Quality control | Restriction Endonucleases: Quality Control |
MSDS: | Download MSDS as PDF |