| Recognition site | CAGNNN↑CTG
GTC↓NNNGAC |
| Source | Pseudomonas stutzeri 217 |
| Assayed on | Lambda DNA |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | Rose | | 25 - 50 | 50 - 75 | 10 - 25 | 25 - 50 | 100 | 100 |
|
| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at -20°C. |
| Ligations | After 10-fold overdigestion with enzyme > 95% of Lambda DNA fragments can be ligated with T4 DNA Ligase and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer Y |
| Methylation sensitivity | not tested |
| Inactivation 20 minutes under | 65oC |
| Notes | When using a buffer other than the
optimal (suppied) SEBuffer, it may be
necessary to add more enzyme to achieve complete digestion.
|
| Quality control | Restriction Endonucleases: Quality Control |
| MSDS: | Download MSDS as PDF |