Product info: HpySE526 I


Name
HpySE526 I
Cat. #E583E584
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition site
ACGT
TGCA
SourceE.coli strain, that carries the cloned HpySE 526I gene from Helicobacter pylori SE526.
Assayed onpUC19 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10075 - 10010 - 2525 - 5010050
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.6); 100 mM NaCl; 0,1 mM EDTA; 200 μg/ml BSA; 1 mM DTT; and 50% glycerol. Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated with T4 DNA Ligase and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivityBlocked by CG methylation
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322