|Source||Sporosarcina species M|
|Assayed on||Lambda DNA|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.|
At 37°C activity is 75% from maximum
|Optimal SE-buffer||Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)|
|Enzyme activity (%)|
|50 - 75||25 - 50||10 - 25||50 - 75||100||80|
|Storage conditions||20 mM Tris-HCl (pH 7.6); 100 mM KCl; 0,1 mM EDTA; 500 μg/ml BSA;0,01% Triton X-100, 7 mM 2-mercaptoethanol, 50% glycerol. |
Store at -20°C.
Storage at -70°C is recommended for periods longer than 30 days.
|Ligation||After 3-fold overdigestion with enzyme 5% of the DNA fragments can be ligated and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer Y|
|Inactivation 20 minutes||No|
|Quality control||Restriction Endonucleases: Quality Control|
|MSDS:||Download MSDS as PDF|
|Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:|
To view the fragments length values please point mouse cursor over diagram
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322