Product info: SspM I


Name
SspM I
Cat. #E591E592
Package, u.a.100500
Concentration, u.a./ml10001000

Recognition site
CTAG
GATC
SourceSporosarcina species M
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
At 37°C activity is 75% from maximum
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
50 - 7525 - 5010 - 2550 - 7510080
Optimal temperature
55oC
Storage conditions20 mM Tris-HCl (pH 7.6); 100 mM KCl; 0,1 mM EDTA; 500 μg/ml BSA;0,01% Triton X-100, 7 mM 2-mercaptoethanol, 50% glycerol.
Store at -20°C.For long term storage (more than 30 days), store at -70°C.
LigationAfter 3-fold overdigestion with enzyme 5% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Inactivation 20 minutes
No
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322