Product info: Lmn I


Name
Lmn I
Cat. #E593E594
Package, u.a.50250
Concentration, u.a./ml500-1000500-1000

Recognition site
GCTCCN
CGAGGN
SourceLysinibacillus manganicus An22
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10075 - 10050 - 7550 - 7575 - 10080
Optimal temperature
37oC
Storage conditions20 mM Tris-HCl (pH 7.5); 250 mM NaCl; 100 μg/ml BSA; 7 mM 2-mercaptoethanol, 50% glycerol. Store at -20ºC.
LigationsAfter 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37ºC.
Reagents Supplied with Enzyme 10 X SE-buffer B
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF