Product info: Ple19 I


Name
Ple19 INot blocked by 
CG(6mA)TCG methylation  
Cat. #E195E196
Package, u.a.100500
Concentration, u.a./ml2000-50002000-5000

Recognition site
CGATCG
GCTAGC
SourcePseudomonas lemoignei 19
Assayed onLambda DNA (HindIII-digest)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10075 - 10025 - 5025 - 50100100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivityNot blocked by overlapping Dam-methylation (GmATC): CGATCG
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Semenchenko, G.V., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations (1994).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322