Product info: Psp124B I


Name
Psp124B I
Cat. #E107E108E107TE108TE107m
Package, u.a.1000500010005000500
Concentration, u.a./ml10000-3000010000-3000010000-3000010000-3000010000

Recognition site
GAGCTC
CTCGAG
SourcePseudomonas species 124B
Assayed onLambda DNA (HindIII-digest)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10010010 - 250 - 1075 - 10030
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer G.
For E107T and E108T an additional 10X SE-buffer ROSE is supplied.
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Degtyarev, S.Kh., Dedkov, V.S., Rechkunova, N.I., Morgan, R. Unpublished observations (1994).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322