Product info: PspE I


Name
PspE I
Cat. #E169E170
Package, u.a.200010000
Concentration, u.a./ml5000-100005000-10000

Recognition site
GGTNACC
CCANTGG
SourcePseudomonas species E
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10050 - 7525 - 5050 - 7550 - 75100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer B
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesHigh enzyme concentration may result in star activity.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Dedkov, V.S., Rechkunova, N.I., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1994).