Product info: PspN4 I Name PspN4 I    Cat. # E089 E090 Package, u.a. 1000 5000 Concentration, u.a./ml 10000 10000 Recognition site GGN↑NCC CCN↓NGG Source Pseudomonas species N4 Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Enzyme activity (%) B G O W Y Rose 10 - 25 10 - 25 10 - 25 25 - 50 100 50 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. Ligation After 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer Y Methylation sensitivity Blocked by 5`-GGNN(5mC)C-3`/3-C(5mC)NNGG-5` or 5`-GGNN(5mC)C-3`/3-CCNNGG-5` methylation Inactivation 20 minutes under 65oC Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (1995).  Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007