|Source||E.coli strain, that carries the cloned gene Dra III from Deinococcus radiophilus|
|Assayed on||Lambda DNA|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.|
|Optimal SE-buffer||2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.) + BSA|
|Enzyme activity (%)|
|25 - 50||50 - 75||75 - 100||75 - 100||50 - 75||100|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.|
|Ligations||After 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut. In the presence of 10%PEG ligation is better.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of DNA
with 10 u.a. of enzyme for 16 hours at 37°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer 2K, BSA|
|Methylation sensitivity||not tested|
|Inactivation 20 minutes under||65oC|
|Notes||High enzyme concentration may result in star activity. |
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
|Quality control||Restriction Endonucleases: Quality Control|
|MSDS:||Download MSDS as PDF|
|References:||de Wit, C.M., Dekker, B.M.M., Neele, A.C., de Waard, A., FEBS Lett. 180: 219-223 (1985)
Grosskopf, R., Wolf, W., Kessler, C., Nucleic Acid res. 13: 1517-1528 (1985)|