Product info: DraIII


Name
DraIII
Cat. #E309E310
Package, u.a.5002500
Concentration, u.a./ml50005000

Recognition site
CACNNNGTG
GTGNNNCAC
SourceE.coli strain, that carries the cloned gene Dra III from Deinococcus radiophilus
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
25 - 5050 - 7575 - 10075 - 10050 - 75100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated and recut. In the presence of 10%PEG ligation is better.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer 2K, BSA
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesHigh enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:de Wit, C.M., Dekker, B.M.M., Neele, A.C., de Waard, A., FEBS Lett. 180: 219-223 (1985) Grosskopf, R., Wolf, W., Kessler, C., Nucleic Acid res. 13: 1517-1528 (1985)