| Recognition site | CAG↑CTG
GTC↓GAC |
| Source | An E.coli strain that carries the cloned Pvu II gene from Proteus vulgaris |
| Assayed on | Lambda DNA |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA |
| Enzyme activity (%) | | B | G | O | W | Y | Rose | | 25 - 50 | 100 | 25 - 50 | 25 - 50 | 25 - 50 | 100 |
|
| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 300 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Ligation | After 10-fold overdigestion with enzyme approximately 70% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer G, BSA
|
| Methylation sensitivity | not tested |
| Inactivation 20 minutes under | 80oC |
| Notes | High enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1 reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
|
| Quality control | Restriction Endonucleases: Quality Control |
| MSDS: | Download MSDS as PDF |
| References: | Repin, V.E., Degtyarev, S.Kh., Unpublished observations.
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
|
