Name | Rsa I | |
| Cat. # | E113 | E114 | Package, u.a. | 1000 | 5000 | Concentration, u.a./ml | 20000 | 20000 |
Recognition site | GT↑AC CA↓TG | Source | Rhodopseudomonas sphaeroides | Assayed on | Lambda DNA | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. | Optimal SE-buffer | B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) | Enzyme activity (%) | B | G | O | W | Y | Rose | 100 | 50 - 75 | 0 - 10 | 50 - 75 | 75 - 100 | 50 |
| Optimal temperature | 37oC | Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol. Store at -20°C. | Ligation | After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
| Reagents Supplied with Enzyme |
10 X SE-buffer B | Methylation sensitivity | not tested | Inactivation 20 minutes | No | Quality control | Restriction Endonucleases: Quality Control | MSDS: | Download MSDS as PDF | References: | Lynn, S.P., Cohen, L.K., Kaplan, S., Gardner, J.F. J.Bacteriol. 142: 380-383 (1980).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
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