| Recognition site | G↑TCGAC
CAGCT↓G |
| Source | An E.coli strain that carries the cloned Sal I gene from Streptomyces albus |
| Assayed on | Lambda DNA (HindIII-digest) |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | Rose | | 0 - 10 | 10 - 25 | 100 | 25 - 50 | 0 - 10 | 5 |
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| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Ligation | After 10-fold overdigestion with enzyme more than 95% of the DNA pUC19 fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
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| Reagents Supplied with Enzyme |
10 X SE-buffer O (E115, E116, E115T, E116T)
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| Methylation sensitivity | not tested |
| Inactivation 20 minutes under | 65oC |
| Notes | High enzyme concentration may result in star activity.
Turbo Sal I can be used for short time (15 min) DNA digestion as well as for standard reaction.
Turbo DNA Digestion:
Applications:
-Fast DNA analysis
-Fast preparation of vectors for cloning
-Double digestion
Enzyme Properties:
1 μl of Turbo Sal I cuts 1 μg of DNA in 1 x SE-Buffer O in 15 min (see the protocol below). A short time of DNA digestion requires high quality purification of DNA sample. The enzyme may be used in standard conditions of DNA cleavage (E115).
Turbo reaction protocol:
20 μl of the reaction volume:
10 x SE Buffer O - 2 μl
DNA - 0,2-1 μg
Nuclease-free water - to 20 μl
+ 1μl of Turbo Sal I
Incubate at 37°C for 15 min.
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| Quality control | Restriction Endonucleases: Quality Control |
| MSDS: | Download MSDS as PDF |
| References: | Arrand, J.R., Myers, P.A., Roberts, R.J., J. Mol. Biol. 118: 113-122 (1978).
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Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
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