Product info: Sal I


Name
Sal I
Cat. #E115E116E115TE116T
Package, u.a.200010000200010000
Concentration, u.a./ml10000100001000010000

Recognition site
GTCGAC
CAGCTG
SourceAn E.coli strain that carries the cloned Sal I gene from Streptomyces albus
Assayed onLambda DNA (HindIII-digest)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
0 - 1010 - 2510025 - 500 - 105
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme more than 95% of the DNA pUC19 fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer O (E115, E116, E115T, E116T)
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesHigh enzyme concentration may result in star activity.
Turbo Sal I can be used for short time (15 min) DNA digestion as well as for standard reaction.
Turbo DNA Digestion:
Applications:
-Fast DNA analysis
-Fast preparation of vectors for cloning
-Double digestion
Enzyme Properties: 1 μl of Turbo Sal I cuts 1 μg of DNA in 1 x SE-Buffer O in 15 min (see the protocol below). A short time of DNA digestion requires high quality purification of DNA sample. The enzyme may be used in standard conditions of DNA cleavage (E115).
Turbo reaction protocol: 20 μl of the reaction volume:
10 x SE Buffer O - 2 μl
DNA - 0,2-1 μg
Nuclease-free water - to 20 μl
+ 1μl of Turbo Sal I
Incubate at 37°C for 15 min.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Arrand, J.R., Myers, P.A., Roberts, R.J., J. Mol. Biol. 118: 113-122 (1978).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322