Product info: AclW I


Name
AclW IBlocked by 
 GG(6mA)TC methylation   
Cat. #E211E212
Package, u.a.100500
Concentration, u.a./ml1000-30001000-3000

Recognition site
GGATC(N)4
CCTAG(N)5
SourceAcinetobacter calcoaceticus W2131
Assayed onLambda DNA (dam-)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
75 - 10050 - 750 - 100 - 1010030
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 2-fold overdigestion with enzyme about 50% of the DNA fragments can be ligated with more than 500 u.a. of DNA ligase.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 6 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Methylation sensitivityBlocked by Dam methylation (GmATC) GGATC
Inactivation 20 minutes under
65oC
NotesTo obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Abdurashitov, M.A., Belichenko, O.A., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations. (1994)
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322