Product info: Sfr274 I


Name
Sfr274 IBlocked by 
 CTCG(6mA)G methylation  Not blocked by 
CT(5mC)GAG methylation  
Cat. #E125E126E125TE126TE125m
Package, u.a.200010000200010000500
Concentration, u.a./ml10000-3000010000-3000010000-3000010000-3000010000

Recognition site
CTCGAG
GAGCTC
SourceStreptomyces fradiae 274
Assayed onLambda DNA (Hind III-digest)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 50°C in a total reaction volume of 50 μl.
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10075 - 10050 - 7550 - 7575 - 100100
Optimal temperature
50oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.. Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 50°C.
Reagents Supplied with Enzyme 10 X SE-buffer B.
For E125T and E126T an additional 10X SE-buffer ROSE is supplied.
Methylation sensitivityBlocked by CTCGmAG methylation.
Not blocked by CTmCGAG methylation.
Inactivation 20 minutes under
65oC
NotesAt 37°C activity is about 70% from maximum.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Puchkova, L.I., Krivopalova, G.N., Andreeva, I.S., Selina, A.V., Serov, G.D., Rechkunova, N.I., Degtyarev, S.Kh. Izv. Sib. Otd. Akad. Nauk SSSR 1: 32-34 (1990).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322