Product info: Sph I


Name
Sph INew package  
Cat. #E129E130E129TE130T
Package, u.a.50025005002500
Concentration, u.a./ml5000500050005000

Recognition site
GCATGC
CGTACG
SourceE.coli strain that carries the cloned Sph I gene from Streptomyces phaeochromogenes
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
25 - 5010075 - 10075 - 10050 - 75100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer G, BSA (except E129T and E130T).
For E129T and E130T an additional 10X SE-buffer ROSE is supplied.
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesTo obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Fuchs, L.Y., Covarrubias, L., Escalante, L., Sanchez, S., Bolivar, F. Gene 10:39-46 (1980).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322