| Recognition site | T↑TAA
AAT↓T |
| Source | E.coli strain that carries the cloned Tru9 I gene from Thermus ruber 9 |
| Assayed on | Lambda DNA |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | Rose | | 75 - 100 | 25 - 50 | 25 - 50 | 100 | 50 - 75 | 100 |
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| Optimal temperature | 65oC |
| Storage conditions | 10 mM Tris-HCl-(pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol Store at -20°C. |
| Ligation | After 20-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 65°C.
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| Reagents Supplied with Enzyme |
10 X SE-buffer W |
| Methylation sensitivity | Blocked by TTAmA methylation . |
| Inactivation 20 minutes under | 80oC |
| Quality control | Restriction Endonucleases: Quality Control |
| MSDS: | Download MSDS as PDF |
| References: | Prichodko, E.A., Rechkunova, N.I., Degtyarev, S.Kh. Sib. Biol. J. 1:57-59 (1991).
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
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Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
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