Product info: Tth111 I


Name
Tth111 I
Cat. #E097E098
Package, u.a.4002000
Concentration, u.a./ml50005000

Recognition site
GACNNNGTC
CTGNNNCAG
SourceE.coli strain, that carries the cloned gene Tth111I from Thermus Thermophilus 111
Assayed onLambda DNA (HindIII-digest)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 65°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10050 - 7510 - 2510 - 25100100
Optimal temperature
65oC
Storage conditions10 mM Tris-HCl (pH 7.5); 500 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
LigationAfter 2-fold overdigestion with enzyme 10% of the DNA fragments can be ligated.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
NotesHigh enzyme concentration may result in star activity.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Shinomiya, T., Sato, S. Nucleic Acids Res. 8: 43-56 (1980).