Recognition site | GACN↑NNGTC CTGNN↓NCAG |
Source | An E.coli strain, that carries the cloned gene Tth111I from Thermus Thermophilus 111 |
Assayed on | Lambda DNA (HindIII-digest) |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 65°C in a total reaction volume of 50 μl. |
Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
Enzyme activity (%) | B | G | O | W | Y | Rose | 75 - 100 | 50 - 75 | 10 - 25 | 10 - 25 | 100 | 100 |
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Optimal temperature | 65oC |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 500 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C. |
Ligation | After 2-fold overdigestion with enzyme 10% of the DNA fragments can be ligated. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 65°C.
|
Reagents Supplied with Enzyme |
10 X SE-buffer Y |
Methylation sensitivity | not tested |
Inactivation 20 minutes under | 80oC |
Notes | High enzyme concentration
may result in star activity.
|
Quality control | Restriction Endonucleases: Quality Control |
MSDS: | Download MSDS as PDF |
References: | Shinomiya, T., Sato, S. Nucleic Acids Res. 8: 43-56 (1980).
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