Product info: Thermolabile Alkaline Phosphatase


Name
Thermolabile Alkaline Phosphatase
Cat. #E365E366
Package, u.a.2001000
Concentration, u.a./ml50005000

SourceIsolated from E.coli strain that carries the cloned Alkaline Phosphatase gene from Alteromonas undina P2
Description Thermolabile Alkaline phosphatase catalyzes the removal of 5' phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.
Unit definitionOne unit is the amount of enzyme that will dephosphorylate 1 μg of pUC19 DNA (linearized with Hind III) in 30 minutes at 25C.
Dephosphorylation is defined as >95% inhibition of recirculation in a self-ligation reaction that is measured by transformation into E.coli.
Reaction bufferSE-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature
25oC
Storage conditions20 mM Tris-HCl (pH 7.6), 0.1 mM ZnCl2, 50% glycerol. Store at -20C
Inactivation 20 minutes under
65oC
Quality controlThe enzyme is purified free of contaminating endonucleases and exonucleases, ribonuclease activities.
MSDS:Download MSDS as PDF