Product info: SP-Taq DNA polymerase


Name
SP-Taq DNA polymerase
Cat. #E333E334
Package, u.a.2001000
Concentration, u.a./ml50005000

SourceIsolated from E.coli strain that carries the cloned Taq DNA polymerase gene from Thermus aquaticus.
DescriptionSP-Taq DNA Polymerase is a fraction of Taq DNA Polymerase which was specially treated and additionally purified. It doesn` t contain PCR detected DNA contaminations. Enzyme is suitable for different manipulations in the field of PCR-diagnostics.
Unit definitionOne unit is the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.
Unit Assay Conditions: 1 x Taq-DNA-polymerase buffer, 200 μM dATP, dCTP, dGTP, 50 mkM H-TTP, 12.5 μg activated Calf Thymus DNA in a total reaction volume of 50 μl.
Reaction bufferSE-buffer DNA polymerase Taq, (60 mM Tris-HCl (pH 8.5 at 25°C); 1.5 mM MgCl2; 25 mM KCl; 10 mM 2-mercaptoethanol; 0.1% Triton X-100.)
Optimal temperature
72oC
Storage conditions20 mM Tris-HCl (pH 7.6); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% Triton X-100; 50% glycerol. Store at -20°C.
Quality control DNA free.
The enzyme is purified free of contaminating endonucleases and exonucleases.
Legal Licenses/Patents/Disclaimers:
Some applications in which this product can be used may be covered by patents issued and applicable in the United States, European Union and certain countries. Because purchase of this product does not include a license to perform any patented application, users of this product may be required to obtain a patent license depending upon the particular application in which the product is used.
MSDS:Download MSDS as PDF