Product info: Vsp I

Vsp IBlocked by ATTA(6mA)T methylation     
Cat. #E139E140E139m
Package, u.a.10005000500
Concentration, u.a./ml100001000010000

Recognition site
SourceE.coli strain, that carries the cloned gene VspI from Vibrio species 343
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
0 - 1010 - 2550 - 7510025 - 5050
Optimal temperature
Storage conditions10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme 70% of the DNA fragments can be ligated.Of these, 90% can be recut. In the presence of 10% PEG ligation is better.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C
Reagents Supplied with Enzyme 10 X SE-buffer W
Methylation sensitivityBlocked by ATTAmAT methylation.
Inactivation 20 minutes under
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Degtyarev, S.Kh., Repin, V.E., Rechkunova, N.I., Tchigikov, V.E., Malygin, E.G., Mikhajlov, V.V., Rasskazov, V.A. Bioorg. Khim. 13: 420-421 (1987).
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322