Product info: Xba I


Name
Xba INew package  Blocked by 
TCTAG(6mA) methylation    
Cat. #E141E142E141XE142XE141TE142TE141m
Package, u.a.200010000200010000200010000500
Concentration, u.a./ml20000200005000050000200002000020000

Recognition site
TCTAGA
AGATCT
SourceE.coli strain, that carries the cloned gene XbaI from Xanthomonas badrii
Assayed onLambda DNA (dam-/HindIII-digest)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-/HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
75 - 10075 - 10010050 - 7575 - 10025
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0,1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer O, BSA (except E141m, E141T and E142T).
For E141T and E142T an additional 10X SE-buffer ROSE is supplied.
Methylation sensitivityBlocked by overlapping Dam-methylation (GmATC): TCTAGATC
Inactivation 20 minutes under
65oC
NotesTo obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Zain, B,S., Roberts, R.J. J. Mol. Biol. 115: 249-255 (1977).
 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322