Product info: Xma I Name Xma I    Cat. # E233 E234 Package, u.a. 300 1500 Concentration, u.a./ml 3000 3000 Recognition site C↑CCGGG GGGCC↓C Source An E.coli strain, that carries the cloned gene XmaI from Xanthomonas malvacearum Assayed on Adenovirus -2 DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus -2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Enzyme activity (%) B G O W Y Rose 75 - 100 50 - 75 0 - 10 0 - 10 100 50 Optimal temperature 37oC Storage conditions 20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C. Ligation After 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated. Of these 90% can be recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37°C Reagents Supplied with Enzyme 10 X SE-buffer Y Methylation sensitivity not tested Inactivation 20 minutes under 65oC Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Endow, S.A., Roberts, R.J. J. Mol. Biol. 112: 521-529 (1977).