Product info: Xma I


Name
Xma INew package  
Cat. #E233E234
Package, u.a.3001500
Concentration, u.a./ml30003000

Recognition site
CCCGGG
GGGCCC
SourceE.coli strain, that carries the cloned gene XmaI from Xanthomonas malvacearum
Assayed onAdenovirus -2 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus -2 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10050 - 750 - 100 - 1010050
Optimal temperature
37oC
Storage conditions20 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 3-fold overdigestion with enzyme 95% of the DNA fragments can be ligated. Of these 90% can be recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 37°C
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Endow, S.A., Roberts, R.J. J. Mol. Biol. 112: 521-529 (1977).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322