Product info: BstEN I


Name
BstEN INot blocked by 
C(5mC)TNNNNNAGG and CCTNNNN(5mc)AGG methylation  
Cat. #E103E104
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

Recognition site
CCTNNNNNAGG
GGANNNNNTCC
SourceBacillus stearothermophillus EM
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
50 - 7550 - 7525 - 5025 - 50100100
Optimal temperature
65oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
LigationsAfter 3-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated. Of these 90% can be recut
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 65°C
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivityNot blocked by overlapping Dcm methylation(CmCWGG): CCTGGNNNAGG,
CCTNNNCCAGG.
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2000).