Product info: BstPA I


Name
BstPA I
Cat. #E299E300
Package, u.a.10005000
Concentration, u.a./ml10000-2000010000-20000

Recognition site
GACNNNNGTC
CTGNNNNCAG
SourceBacillus stearothermophillus PA
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
50 - 7525 - 5050 - 7550 - 75100100
Optimal temperature
65oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme less than 5% of the DNA fragments can be ligated
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 25°C
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivitynot tested
Inactivation 20 minutes
No
NotesHigh enzyme concentration and incubation at 65°C for 16 hours results in star activity.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Tomilova, J.E., Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.Kh. Unpublished observations (2000).