Product info: Ahl I


Name
Ahl I
Cat. #E173E174E173TE174T
Package, u.a.1000500010005000
Concentration, u.a./ml10000-3000010000-3000010000-3000010000-30000

Recognition site
ACTAGT
TGATCA
SourceAlteromonas haloplanktis SP
Assayed onT7 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
10075 - 10025 - 5025 - 5075 - 100100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme more than 90% of T7 DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer B, BSA (except E173T and E174T).
For E173T and E174T an additional 10X SE-buffer ROSE is supplied.
Methylation sensitivitynot tested
Inactivation 20 minutes
No
NotesTo obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Myakisheva, T.V., Belichenko, O.A., Popichenko, D.V., Dedkov, V.S., Degtyarev, S.Kh. Unpublished observations (2000).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322