Product info: BstHH I

BstHH IBlocked by 
5`-G(5mC)GC-3`/3-CG(5mC)G-5` or 
methylation   Not blocked by 
5`-GCG(5mC)-3`/3`-(5mC)GCG-5` or 
Cat. #E143E144
Package, u.a.200010000
Concentration, u.a./ml5000050000

Recognition site
SourceBacillus stearothermophilus HH
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA
Enzyme activity (%)
75 - 10050 - 7525 - 5050 - 75100100
Optimal temperature
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA, 50% glycerol; Store at 20°C
LigationAfter 40-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of DNA with 100 u.a. of enzyme for 16 hours at 65°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Methylation sensitivityBlocked by
5`-G(5mC)GC-3`/3-CG(5mC)G-5` or
5`-G(5mC)GC-3`/3`-CGCG-5` methylation.
Not blocked by
5`-GCG(5mC)-3`/3`-(5mC)GCG-5` or
5`-GCG(5mC)-3`/3`-CGCG-5` methylation.
Inactivation 20 minutes
NotesTo obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Shapovalova, M.A., Abdurashitov, M.A., Tumanova, I.Y., Dedkov, V.S., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).