Product info: Bpu10I


Name
Bpu10I  
Cat. #E149E150
Package, u.a.2001000
Concentration, u.a./ml50005000

Recognition site
CCTNAGC
GGANTCG
SourceE.coli strain, that carries recombinant plasmids
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferK (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM KCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10 - 2525 - 5050 - 7550 - 7525 - 50100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C
LigationsAfter 5-fold overdigestion with enzyme 80% of the DNA fragments can be ligated. Of these 90% can be recut. In the presence of 10% PEG ligation is better.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 1 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer K
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
NotesHigh enzyme concentration may result in star activity or incomplete DNA cleavage. We recommend increasing incubation time instead of using an excess of Bpu10I.
The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,5.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Degtyarev, S.Kh., Zilkin, P.A., Prihodko, G.G., Repin, V.E., Rechkunova, N.I. Mol. Biol. (Mosk) 23: 1051-1056 (1989).
 Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006