Product info: BstC8I

BstC8Inew package  
Cat. #E305E306
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition site
SourceBacillus stearothermophilus C8
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
10 - 2525 - 5050 - 7575 - 100100100
Optimal temperature
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 10-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 55°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivityBlocked by GC methylation: 5`-G(5mC)NNGC-3`/3`-CGNN(5mC)G-5`.
Inactivation 20 minutes under
NotesAt 37°C activity is 50% from maximum.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Abdurashitov, M.A., Dedkov, V.S., Mikhnenkova, N.A., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).
Dedkov, V.S. Novel M.BstC8I Methyltransferase Forms 5'-Gm5CNNGC-3'. Investigation of Restriction Endonuclease Sensitivity to M.BstC8I Methylation. // Mol. Gen., Microbiol. and Virology, Vol. 27. No.1. P. 40-47 (2012).