Name | BstSCI | |
| Cat. # | E307 | E308 | Package, u.a. | 100 | 500 | Concentration, u.a./ml | 2000-5000 | 2000-5000 |
Recognition site | ↑CCNGG GGNCC↓ | Source | Bacillus stearothermophilus SC | Assayed on | Lambda DNA (dcm-) | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 55°C in a total reaction volume of 50 μl. | Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) | Enzyme activity (%) | B | G | O | W | Y | Rose | 50 - 75 | 50 - 75 | 50 - 75 | 50 - 75 | 100 | 60 |
| Optimal temperature | 55oC | Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol. Store at -20°C. | Ligation | After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut. | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 55°C.
| Reagents Supplied with Enzyme |
10 X SE-buffer Y | Methylation sensitivity | Blocked by Dcm-methylation (CmCWGG) CCWGG. | Inactivation 20 minutes under | 80oC | Notes | At 37°C activity is 10% from maximum.
| Quality control | Restriction Endonucleases: Quality Control | MSDS: | Download MSDS as PDF | References: | Dedkov, V.S., Muradov, S.V., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K.
Unpublished observations (2001).
Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
|
| |