Product info: BstSCI


Name
BstSCIBlocked by 
C(5mC)NGG methylation    
Cat. #E307E308
Package, u.a.100500
Concentration, u.a./ml2000-50002000-5000

Recognition site
CCNGG
GGNCC
SourceBacillus stearothermophilus SC
Assayed onLambda DNA (dcm-)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dcm-) in 1 hour at 55°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
50 - 7550 - 7550 - 7550 - 7510060
Optimal temperature
55oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; and 50% glycerol. Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 3 u.a. of enzyme for 16 hours at 55°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivityBlocked by Dcm-methylation (CmCWGG) CCWGG.
Inactivation 20 minutes under
80oC
NotesAt 37°C activity is 10% from maximum.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Dedkov, V.S., Muradov, S.V., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
 Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007