Product info: BstV1I


Name
BstV1I
Cat. #E303E304
Package, u.a.100500
Concentration, u.a./ml1000-20001000-2000

Recognition site
GCAGC(N)8
CGTCG(N)12
SourceBacillus stearothermophilus V1
Assayed onpBR322 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Optimal SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10010075 - 10075 - 10075 - 100100
Optimal temperature
55oC
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
LigationsAfter 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 55°C.
Reagents Supplied with Enzyme 10 X SE-buffer G
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
NotesAt 37°C activity is 10% from maximum.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Dedkov, V.S., Muradov, S.V., Shinkarenko, N.M., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322