Product info: BstKTI


Name
BstKTIBlocked by 
G(6mA)TC methylation  Not blocked by 
GAT(5mC) methylation  
Cat. #E151E152
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

Recognition site
GATC
CTAG
SourceBacillus stearothermophilus KT
Assayed onLambda DNA (dam-)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
25 - 5050 - 7575 - 10010050 - 75100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer W
Methylation sensitivityBlocked by overlapping Dam methylation (GmATC) GATC .
Not blocked by CG methylation.
Cut hemimethylated site: 5`- GmATC-3` / 5`-GATC-3`
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Nadeev A.N., Chernukhin V. A., Sevastyanova O.O., Tomilova J.E., Shinkarenko N.M., Evdokimov A.A., Degtyarev S. Kh. BstKTI, a New dam-Sensitive Neoschizomer of Restriction Endonuclease MboI, which is Able to Cleave Hemimethylated Substrate.// Biotekhnologia (Moscow), No.2, 5-10 (2006). (In Russian)
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322